Search results for "Antibody-Secreting Cells"

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Antibody synthesis in roach (Rutilus rutilus); analysis of antibody secreting cells in lymphoid organs with ELISPOT-assay

1994

The roach (Rutilus rutilus L.) which is a cyprinid fish, was immunised with bovine γ-globulin (BGG) and the antibody synthesis was studied by counting the number of specific antibody secreting cells (SASC) in the spleen and anterior kidney, and by measuring the antibody concentration in the circulation. SASCs and the total number of immunoglobulin secreting cells (TISC) were counted with the ELISPOT (enzyme-linked immunospot) assay, and anti-BGG antibodies and the concentration of immunoglobulin in sera were assayed by ELISA (enzyme-linked immunosorbent assay). The present modification of the ELISPOT-assay takes advantage of biotin-avidin amplification and yields easily detectable and nonfa…

KidneyELISPOTSpleenGeneral MedicineAquatic ScienceBiologybiology.organism_classificationSpecific antibodymedicine.anatomical_structureLymphatic systemImmunologymedicineAntibody-Secreting Cellsbiology.proteinEnvironmental ChemistryRutilusAntibodyFish & Shellfish Immunology

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